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Agricultural & Environmental Letters Abstract - Research Letters

Cryptosporidium Soil Extraction by Filtration/IMS/FA Compatible with USEPA Method 1623.1

 

This article in AEL

  1. Vol. 1 No. 1 160031
    unlockOPEN ACCESS
     
    Received: Aug 26, 2016
    Accepted: Nov 10, 2016
    Published: December 15, 2016


    * Corresponding author(s): zopp@wisc.edu
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doi:10.2134/ael2016.08.0031
  1. Zachariah P. Zopp *a,
  2. Jeremy M. Olstadtb,
  3. K. G. Karthikeyana,
  4. Anita M. Thompsona and
  5. Sharon C. Longc
  1. a Dep. of Biological Systems Engineering, Univ. of Wisconsin-Madison, 460 Henry Mall, Madison, Madison, WI, 53706
    b Dep. of Water Microbiology, Wisconsin State Laboratory of Hygiene, 2601 Agriculture Dr., Madison, WI, 53718
    c Dep. of Soil Sciences, Univ. of Wisconsin-Madison, 1525 Observatory Drive, Madison, WI, 53706
Core Ideas:
  • Cryptosporidium extraction from the soil matrix is absent in USEPA Method 1623.1.
  • Our S3PEG soil extraction is compatible with USEPA Method 1623.1.
  • S3PEG extraction meets quality control recoveries specified by USEPA Method 1623.1.
  • Irradiated Cryptosporidium oocysts are suitable soil surrogate for live oocysts.

Abstract

Cryptosporidium, a parasitic pathogen, when present in drinking water supplies poses a significant risk to human health. The soil environment provides a critical linkage between Cryptosporidium parent material (bovine manure) and the surface and groundwaters used by susceptible human populations for drinking water. The lack of a USEPA published analysis for the determination of Cryptosporidium in soil is a barrier to standardized investigation of Cryptosporidium transport in soils. Methodologies have been published in the absence of a USEPA analysis, yet they lack sufficient detail and quality controls to be generally applicable. This study developed and evaluated a Cryptosporidium soil extraction method capable of producing an extracted fluid containing Cryptosporidium that could be purified and enumerated using USEPA Method 1623.1.

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Copyright © 2016. . Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.