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This article in AJ

  1. Vol. 105 No. 6, p. 1565-1570
     
    Received: Jan 09, 2013
    Published: August 30, 2013


    * Corresponding author(s): mcpilar@ubu.es
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doi:10.2134/agronj2013.0010

Barley Seeds Encapsulated in Calcium-Alginate Gels with Phosphatase and Humate-Phosphatase Complexes for Improving Phosphorus Bioavailability

  1. María Concepción Pilar-Izquierdo *a,
  2. María D. Bustoa,
  3. Natividad Ortegaa and
  4. Manuel Perez-Mateosa
  1. a Dep. of Biotechnology and Food Science, Area of Biochemistry and Molecular Biology, Univ. of Burgos, Plaza Misael Bañuelos, s/n, e-09001 Burgos, Spain

Abstract

A large portion of the soluble P fertilizers introduced into soil reacts with soil components forming insoluble P products, which are not efficiently taken up by plants. Barley seed (Hordeum vulgare L. ‘Volley’) encapsulation in Ca-alginate gels containing phosphatase free or immobilized with soil humates (humate–phosphatase complexes, HPC) was investigated as a tool to enhance the utilization by plants of accumulated soil P. The effect of the enzyme/carboxymethylcellulose solution ratio on the coating phosphatase activity and on seed germination was studied. A pot experiment with encapsulated seeds was conducted. Dry weight, length and shoot P content were estimated at different days after planting (DAP). Under optimal conditions (0.20 mL mL–1 free phosphatase and 0.13 g mL–1 HPC), the seed encapsulation with free phosphatase and HPC resulted in a coating phosphatase activity of 20 and 17% and in germination percentages of 92 and 85%, respectively. Increased phosphatase activity was observed in the rhizosphere of encapsulated seeds in comparison with non-treated ones. Under pot culture conditions, at 35 DAP, the inorganic P in soil planted with encapsulated seeds was higher (14–16%) than in control soil. In contrast, soil planted with treated seeds showed lower content of organic P (20–29%) than the control soil. Furthermore, the seed encapsulation significantly increased the shoot P content and the P uptake between 24 and 28% and 15 to 20%, respectively, at 35 DAP. In conclusion, seeds treated with phosphatase could substantially contribute to enhance the plant P nutrition in the early stages of seedling establishment.

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Copyright © 2013. Copyright © 2013 by the American Society of Agronomy, Inc.