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This article in AJ

  1. Vol. 62 No. 1, p. 36-39
    Received: Mar 3, 1969

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Adaptation of Gas-Liquid Chromatography to Analysis of Certain Sugars of Cotton Leaves1

  1. Wayne E. Sabbe and
  2. George W. Cathey2



A procedure involving gas-liquid chromatography (GLC) of certain sugars in cotton leaves was studied. The use of hexamethyl-disilazane (HMDS) and trimethylchlorosilane (TMS) for silylation of fructose, glucose, and sucrose gave recovery percentages ranging from 71 to 105%. The substitution of trifluoracetic acid (TFA) for TMS gave recovery percentages of 91 to 109%. The use of TFA also improves the ease of sample preparation since absolute exclusion of water was not necessary. Details of sample preparation and operating parameters of GLC are given.

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