Adaptation of Gas-Liquid Chromatography to Analysis of Certain Sugars of Cotton Leaves1
- Wayne E. Sabbe and
- George W. Cathey2
A procedure involving gas-liquid chromatography (GLC) of certain sugars in cotton leaves was studied. The use of hexamethyl-disilazane (HMDS) and trimethylchlorosilane (TMS) for silylation of fructose, glucose, and sucrose gave recovery percentages ranging from 71 to 105%. The substitution of trifluoracetic acid (TFA) for TMS gave recovery percentages of 91 to 109%. The use of TFA also improves the ease of sample preparation since absolute exclusion of water was not necessary. Details of sample preparation and operating parameters of GLC are given.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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