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Agronomy Journal Abstract -

Method for Estimation of Tannin in Grain Sorghum1


This article in AJ

  1. Vol. 63 No. 3, p. 511-512
    Received: Oct 17, 1970

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  1. Robert E. Burns2



The acidified vanillin method of tannin analysis, widely used for forage crops, was modified to permit rapid estimation of relative tannin content of grain sorghum [Sorghum bicolor (L.) Moench]. The method involves overnight extraction of ground grain with methanol at room temperature. An aliquot of the extract is added to a solution of vanillin and hydrochloric acid and the resultant color is read on a colorimeter at 500-525 mµ The reagent is specific for astringent compounds in plants and the rcsuhs were highly correlated with digestibility.

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