Variation of Soluble Phenolic Compounds in Flue-cured Tobacco Cultivars with Stalk Position1
- R. A. Andersen,
- T. C. Tso and
- J. F. Chaplin2
Phenolic levels in field-grown flue-cured leaves of ‘Coker 139,’ ‘NC 95,’ ‘SC 58,’ and ‘LN 38’ were compared within each cultivar according to leaf positions on the stalks and among the four cultivars. Each of the cultivars showed a significant but generally nonlinear increase in levels of total phenolics (about 2-fold for each) and sums of chlorogenic acids and rutin (2 to 9-fold depending on cultivar) with eight ascending leaf positions on the stalk from lower to upper leaves. However, mean values of total phenolic reducing power equivalent to a chlorogenic acid standard and averaged over all stalk positions ranged from 7.17% in Coker 139 to 7.92% in NC 95; these values were not significantly different among the cultivars. Likewise, means of the sums of chlorogenic acids and rutin averaged over all leaf positions on the stalk ranged from 120% in Coker 139 to 1.59% in NC 95, and the four cultivars were not significantly different. Total soluble phenolic compounds were estimated by their bonding to insoluble polyvinylpyrrolidone at pH 3.5. Chlorogenic acid isomers and rutin were determined by electron capture gas chromatographlc analysis based upon the amount of soluble caffeic acid and quercetin moieties found. Leaf phenolic compounds affect color and quality and may contribute to the tumor-promoting weak acid fraction of tobacco (Nicotiana tabacum L.) smoke condensate. Therefore, leaves of these cultivars from different positions on the stalk will be used for cigarette manufacture to correlate the phenolic-stalk position findings with smoke characteristics and composition.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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