Modified Milling Procedure for Separating Endosperm and Nonendosperm Portions of the Wheat Kernel for Protein and Lysine Analysis1
- K. P. Vogel,
- P. J. Mattern and
- G. W. Lenser2
Conventional milling does not uniformly separate endosperm and nonendosperm components of wheats (Triticum aestivum L.). Uniform separation of bran and endosperm is required for protein and lysine analyses because of a protein gradient in wheat endosperm. A modified milling procedure for separating endosperm and nonendosperm components of the wheat kernel was evaluated for its utility in processing small breeder lots of seed for protein and lysine analyses. After conventional milling and sifting, the endosperm adhering to the bran was removed by washing with a 80:20 (vol/vol) ethanol:acetone solution, filtered from the washing solution, dried, and added to the mill flour to reconstitute the endosperm. Reconstituted endosperm weight was used to calculate percent endosperm. Reasonably complete and uniform separations of the endosperm and nonendosperm components were obtained without any appreciable loss of components. Endosperm percentages of the wheats tested ranged from 72.8 to 85.5%. Endosperm protein percentages ranged from 10.8 to 21.0%. Bran protein percentages ranged from 13.1 to 25.1%. Endosperm lysine (percent of protein) percentages ranged from 2.1 to 3.1%. Bran lysine (percent protein) percentages ranged from 3.2 5.0%.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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