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This article in AJ

  1. Vol. 72 No. 6, p. 923-928
    Received: Aug 25, 1978

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Imbibitional Chilling Injury in Soybean Axes: Relationship to Stelar Lesions and Seasonal Environments1

  1. E. N. Ashworth and
  2. R. L. Oberdorf2



The increasing use of isolated embryonic axes as experimental issues for studies on early germination events warrants an evaluation of stress effects during hydration. The objectives were to evaluate embryonic axis growth and stress crack formation in soybean (Glycine max (L.) Merr.) seeds and isolated axes resulting from hydrational stresses of cold temperatures and low tissue moisture. Sensitivity to imbibitional chilling of seeds and isolated axes was evaluated in several cultivars produced in different years at different locations. Germination, seedling growth, and formation of stelar lesions were measured. Isolated axes with an initial moisture content of 6% were injured by imbibition at 5 C as evidenced subsequently by reduced growth at 25 C. Adjustment of axis moisture to 17% before hydration protected against imbibitional chilling injury. Sensitivity to imbibitional chilling was influenced by cultivar and year or location of production. Lesions formed across the stele of hypocotyls during rapid hydration of 6% initial moisture isolated axes at 5 or 25 C but were not observed after hydration of 17% moisture axes or after hydration of intact seeds. The presence of lesions suggests caution when using isolated embryonic axes as experimental tissues.

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