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Agronomy Journal Abstract -

Induction of Tobacco Seed Germination Synchrony through Dark Preincubation1


This article in AJ

  1. Vol. 79 No. 3, p. 468-472
    Received: Mar 28, 1986

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  1. S. C. Mohapatra,
  2. J. Arcila,
  3. W. H. Johnson and
  4. L. A. Nelson2



Nicotiana tabacum cv. Speight G-28, a popular cultivar of bright leaf tobacco, has a high percentage of seed germination but it takes a long time (nearly a week under laboratory conditions and several weeks under field conditions) to give maximum germination. This study was undertaken to develop a laboratory seed treatment to improve germination synchrony. Twenty-three seed treatments, including the control, were developed through the use of dark-preincubation (DPI), polyethylene glycol (PEG) or chilling alone, or in various combinations thereof. An index of synchrony (IS) was calculated from the formula, IS = highest percentage of seeds germinating within a 24-h period / final germination percentage. The theoretical highest value of the IS for a given seed lot would be 1.0. In absence of an absolute synchrony, the practical objective of a given seed treatment would be to raise the IS as close to 1.0 as possible. All the 23 seed treatments tested were ranked on the basis of this consideration. Treatments giving 0.8 or greater IS are, indecreasing order: DPI at 23°C for 1 week followed by DPI for 1 week under chilling (0.95); DPI under chilling for 1 week followed by DPI at 23°C for 1 week (0.93); DPI at 23°C for 1 week followed by chilling under light for 1 week (0.90); and DPI at 23°C for 1 week (0.81). As compared to these treatments, the IS of the control was 0.52. Possible physiological mechanisms involved in DPI-induced germination synchrony and the potential for practical application of this treatment are discussed.

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