A Hot-Wire Technique for Girdling Stems and Petioles of Herbaceous Plants1
- Steven A. Dewey,
- David O. Chilcote and
- Arnold P. Appleby2
Girdling is often useful in studying translocation of herbicides, plant-growth regulators, and natural plant components. A hot-wiretechnique for girdling petioles and stems of herbaceous broadleaf plants using two girdling instruments was studied. One apparatus used AC current to heat a loop in a copper wire. Current was regulated with a rheostat. The other device was a battery-powered medical cautery instrument with a modified nichrome wire loop. With either device, the hot wire was passed back and forth along a 20- to 30-mm section of petiole or stem until cellular collapse was evident, usually 30 to 60 s. Girdling essentially eliminated basipetal movement of 14C-assimilate but did not reduce acropetal translocation of simazine (6-chloro-AyV-diethyl-l,3,5-triazine-2,4-diamine), an apoplastically mobile herbicide, in tall morningglory [Ipomoea purpurea (L.) Roth]. The method is quick and allows precise placement of girdlesPlease view the pdf by using the Full Text (PDF) link under 'View' to the left.
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