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This article in AJ

  1. Vol. 81 No. 6, p. 906-910
     
    Received: July 5, 1988


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doi:10.2134/agronj1989.00021962008100060012x

Image Analysis Morphometric Measurement for Tissue Water Status and Other Determinations

  1. L. Art Spomer  and
  2. M. A. L. Smith
  1. Dep. of Horticulture, 1201 S. Dorner Dr., Univ. of Illinois, Urbana, IL 61801.

Abstract

Abstract

Agronomic research often requires observations involving accurate quantification of plant morphometric properties. Conventional methods are usually burdensome and often inaccurate, especially when features of interest are irregular in shape. A video image analysis system capable of accomplishing rapid and accurate morphometric measurements was demonstrated. The degree of shrinking or swelling of leaf disks was determined of chrysanthemum (Chrysanthemum morifolium Ramat.), soybean [Glycine max (L.) Merr.], and pothos (Epipremnum aureum) in an isopiestic evaluation of tissue water status. This image analysis setup, with a potential resolution of 2.357 ✕ 10−6 cm2, rapidly (about 20 s) resolved minute disk area changes within a range of 0.2463 to 0.2827 cm2, with a maximum deviation of 1.5 X 10−4 cm2 (approximately 0.057% of disk total area). Microcomputerized video image analysis is concluded to be a valuable tool for achieving previously impossible or difficult morphometric measurements in a wide range of experiments.

Contribution of the Illinois Agric. Exp. Stn. (projects 65-353 and 65-363). This work supported in part by the Fred Gloeckner Foundation, Horticulture Res. Inst., The Lilly Endowment, and IBM Corp.-Univ. Illinois ACIS/AEP Project EXCEL.

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