Image Analysis Morphometric Measurement for Tissue Water Status and Other Determinations
- L. Art Spomer and
- M. A. L. Smith
Agronomic research often requires observations involving accurate quantification of plant morphometric properties. Conventional methods are usually burdensome and often inaccurate, especially when features of interest are irregular in shape. A video image analysis system capable of accomplishing rapid and accurate morphometric measurements was demonstrated. The degree of shrinking or swelling of leaf disks was determined of chrysanthemum (Chrysanthemum morifolium Ramat.), soybean [Glycine max (L.) Merr.], and pothos (Epipremnum aureum) in an isopiestic evaluation of tissue water status. This image analysis setup, with a potential resolution of 2.357 ✕ 10−6 cm2, rapidly (about 20 s) resolved minute disk area changes within a range of 0.2463 to 0.2827 cm2, with a maximum deviation of 1.5 X 10−4 cm2 (approximately 0.057% of disk total area). Microcomputerized video image analysis is concluded to be a valuable tool for achieving previously impossible or difficult morphometric measurements in a wide range of experiments.
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