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Book: Proceedings of the Second International Turfgrass Research Conference
Published by: American Society of Agronomy, Crop Science Society of America



  1.  p. 500-507
    Proceedings of the Second International Turfgrass Research Conference

    Eliot C. Roberts (ed.)

    ISBN: 978-0-89118-573-4


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Influence of mineral nutrition on the cold tolerance and soluble protein fraction of centipedegrass1

  1. H. D. Palmertree,
  2. C. Y. Ward and
  3. R. H. Pluenneke


A prepared soil, purposely low in the major plant nutrients, was established to a solid sod of centipedegrass, Eremochloa ophiuroides (Munro) Hack, under field conditions. During Summer 1971, plots of the centipedegrass turf were fertilized with 0.98, 1.96, and 2.94 kg of N; 0.49, 0.98, and 1.47 kg of P2O5; and 0.98, 1.96, and 2.94 kg of K2O/100 m2 in a factorial arrangement. In midfall, prior to the first frost, a portion of the sod and soil was removed intact from each plot and transferred to a greenhouse and grown as unhardened sod. The sod remaining in the field plots was designated as hardened sod.

Sod portions from each fertility treatment of both hardened and unhardened centipedegrass were subjected to low temperature freezing studies to determine the minimum critical temperature for centipedegrass. Centipedegrass which had been fertilized with N rates greater than 1.96 kg/100 m2 was significantly less tolerant of low temperatures. Varying rates of P and K had no effect on cold damage in the low temperature chamber, but under field conditions, K at 1.96 and 2.94 kg/100 m2 increased winter survival of centipedegrass compared to the lower rate of K. Under field conditions, 1.96 kg of N was needed to maintain turf quality and this rate of N actually gave a slight increase in winterhardiness over the lower rate. No difference in the low temperature tolerance was found between field-hardened and unhardened greenhouse sod, suggesting that this grass might obtain maximum hardiness with shortened photoperiod.

Polyacrylamide gel electrophoresis of extracts from centipedegrass leaves revealed that the number of soluble protein bands decreased upon hardening, while the number of soluble protein bands in gels loaded with extract from stolons and crowns increased with hardening. Gels from leaf tissue extract contained more bands than did the gels containing the soluble protein from stolons and crowns. The total soluble protein concentration in stolons and crowns was increased by low temperature hardening. Total soluble proteins also increased with increased N fertilization up to the 1.96 kg rate, but decreased significantly when 2.94 kg of N were applied. Additional index words: Cold hardiness, Soluble proteins, Electrophoresis, Centipedegrass.

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Copyright © 1974. Copyright 1974 by the American Society of Agronomy, Inc. and the Crop Science Society of America, Inc., 5585 Guilford Rd., Madison, WI 53711 USA