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Book: Genetics A Laboratory Manual, 2009
Published by: American Society of Agronomy, Crop Science Society of America

 

Students will learn the experimental aspects of genetics through 15 three-hour laboratory exercises with bacterial, plant, and animal organisms. Each exercise consists of background information, a material list, a step-by-step procedure, and study questions. Learning objectives include an understanding of chromosomes, the physical properties of DNA, DNA digestion and construction of restriction maps, methods of DNA extraction, gene amplification, advanced techniques of gene cloning, Southern blotting, regulation of gene expression, transmission genetics, analysis of crossing over, complementation test, the use of molecular markers in gene mapping, and population genetics. Appendices address class reports, writing scientific articles, and a list of suppliers. In this edition, all chapters have been updated; new exercises have been included in the chapters dealing with DNA extraction and gene expression. The topic of transmission genetics has been enriched with an exercise in sex-linked inheritance, and the concept of natural selection has been developed in the population genetics exercises. New protocols have been added to selected chapters to give advice in maintaining stocks for future use.

Table of Contents




    • Front Matter
      doi:10.2135/2009.geneticslabmanual.frontmatter
      Genetics A Laboratory Manual, I-V
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    • Gregore Koliantz and Daniel B. Szymanski
      Cell Division(Mitosis): Looking at Chromosomes
      doi:10.2135/2009.geneticslabmanual.c1
      Genetics A Laboratory Manual, 1-5
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    • Gregore Koliantz and Daniel B. Szymanski
      Physical Properties of DNA and DNA Assay
      doi:10.2135/2009.geneticslabmanual.c2
      Genetics A Laboratory Manual, 6-12
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    • Gregore Koliantz and Daniel B. Szymanski
      Restriction Testing
      doi:10.2135/2009.geneticslabmanual.c3
      Genetics A Laboratory Manual, 13-18
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    • Gregore Koliantz and Daniel B. Szymanski
      Extraction of Genomic DNA
      doi:10.2135/2009.geneticslabmanual.c4
      Genetics A Laboratory Manual, 19-22
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    • Gregore Koliantz and Daniel B. Szymanski
      Polymerase Chain Reaction
      doi:10.2135/2009.geneticslabmanual.c5
      Genetics A Laboratory Manual, 23-31
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    • Gregore Koliantz and Daniel B. Szymanski
      Cloning 1 Generating Recombinant Plasmids
      doi:10.2135/2009.geneticslabmanual.c6
      Genetics A Laboratory Manual, 32-36
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    • Gregore Koliantz and Daniel B. Szymanski
      Cloning 2 Minipreping
      doi:10.2135/2009.geneticslabmanual.c7
      Genetics A Laboratory Manual, 37-40
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    • Gregore Koliantz and Daniel B. Szymanski
      Southern Blotting 1 DNA Transfer
      doi:10.2135/2009.geneticslabmanual.c8
      Genetics A Laboratory Manual, 41-43
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    • Gregore Koliantz and Daniel B. Szymanski
      Southern Blotting 2 DNA–DNA Hybridization and Sequence Detection
      doi:10.2135/2009.geneticslabmanual.c9
      Genetics A Laboratory Manual, 44-50
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    • Gregore Koliantz and Daniel B. Szymanski
      Regulation of Gene Expression
      doi:10.2135/2009.geneticslabmanual.c10
      Genetics A Laboratory Manual, 51-61
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    • Gregore Koliantz and Daniel B. Szymanski
      Transmission Genetics (Heredity)
      doi:10.2135/2009.geneticslabmanual.c11
      Genetics A Laboratory Manual, 62-76
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    • Gregore Koliantz and Daniel B. Szymanski
      Meiosis and Analysis of Crossing Over
      doi:10.2135/2009.geneticslabmanual.c12
      Genetics A Laboratory Manual, 77-83
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    • Gregore Koliantz and Daniel B. Szymanski
      Complementation Test
      doi:10.2135/2009.geneticslabmanual.c13
      Genetics A Laboratory Manual, 84-89
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    • Gregore Koliantz and Daniel B. Szymanski
      Molecular Markers: Mapping the Genome of Arabidopsis
      doi:10.2135/2009.geneticslabmanual.c14
      Genetics A Laboratory Manual, 90-96
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    • Gregore Koliantz and Daniel B. Szymanski
      Population Genetics How Changes Occur within a Population
      doi:10.2135/2009.geneticslabmanual.c15
      Genetics A Laboratory Manual, 97-101
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