Understanding pasture responses to urine deposition remains limited. From 2011 to 2012, we investigated the effects of urine collected from ruminants and applied to N-fertilized (0, 40, 80, 120, and 160 lb N/acre) plots (25 sq ft) of smooth bromegrass (Bromus inermis Leyss.) in eastern Nebraska. Urine was spread evenly across plots at both a volume (0.6 qt/sq ft) and N content (0.26 and 0.24 oz/qt) that simulated ruminant urine deposition in spring and resulted in an additional supply of 425 and 392 lb N/acre in 2011 and 2012, respectively. Control plots received the same volume of distilled water but no additional N. Response variables included forage mass, crude protein (CP) concentration, N uptake, apparent N recovery (ANR), and N use efficiency (NUE). In 2011, forage mass, CP, and N uptake increased linearly with N fertilizer rate and were 80, 30, and 135%, respectively, greater in urine-treated than in distilled water–treated plots. In 2012, drought limited forage mass, and only urine impacted N responses. Apparent N recovery averaged 42% in urine-treated and 63% in distilled water–treated plots in 2011 but did not differ between treatments in 2012, averaging 24%. Values for NUE did not differ among treatments and averaged 13.0 lb dry matter (DM)/lb N fertilizer applied in 2011 and 6.5 lb DM/lb N fertilizer applied in 2012. In years with favorable precipitation, producers can expect forage mass and CP to increase with fertilizer applications up to 160 lb N/acre. Optimum rates, though, would depend on production goals, nutritional needs of livestock, and fertilizer, land, and cattle prices.