Serological Evaluation of Winter Wheat Aneuploids1
- R.B. Volin and
- J.R. Welsh2
Antigenic components from the leaves of several monosomic (2n - 1) and disomic (2n) plants from each of 18 monosomic lines of winter wheat, Triticum aestivum L. em. Thell. (aestivum group) ‘Kharkof MC22’ (2n = 42), were compared serologically.
Antibodies were produced in rabbits by injecting them with leaf extract antigens from two sources: (1) a disomic which was derived from segregating monosomic and disomic lines of the variety Kharkof MC22 and (2) a single plant selection from Kharkof MC22. Two lots of crude sap and two lots treated with ammonium sulfate saturation methods were used.
Gel-diffusion techniques revealed no differences between the different antigens or between the monosomic and disomic antigens when reacted against any one antiserum. Precipitation lines of partial or non-identity were not present. No antibodies remained following antiserum absorption with monosomic plant extract.
No differences could be found between antigens inherent in the monosomic and disomic genetic conditions when compared serologically.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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