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This article in CS

  1. Vol. 10 No. 5, p. 503-506
    Received: Jan 28, 1970

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Uterotropic Activity and Heritability of Biochanin A and Formononetin in Trifolium medium L.1

  1. L. M. Gourley,
  2. W. F. Keim and
  3. Martin Stob2



The four isoflavones, genistein, biochanin A, formononetin, and daidzein, were identified in zigzag clover, Trifolium medium L. Biochanin A and formononetin were the predominant isoflavones; genisteln and daidzein were present only in trace amounts. The isoflavones were separated by thin-layer chromatography and quantified spectrophotometrically.

Biochanin A and formononetin concentrations were established for samples collected from two greenhouse and two field harvests of a complete six-clone diallel cross. Analysis of variance by Griffing's combining-ability procedure indicated that the significant genetic variance for the isoflavones was primarily explained by general combining ability.

The upper leaflets of zigzag clover contained about 1/3 more biochanin A and formononetin than the lower ones, while the internodes contained about 1/10 as much formononetin as the leaflets, and only a trace of biochanin A.

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