Fluorometric Assay of Free And Bound, cis- and trans-σ-Hydroxycinnamic Acid in a Single Plant Extract1
- F. A. Haskins and
- H. J. Gorz2
Fluorescence is measured on unhydrolyzed and hydrolyzed plant extracts, with and without prior ultraviolet treatment. Readings permit direct determination of free σ-hydroxycinnamic acid (σ-HCA), free trans-σ-HCA, total σ-HCA, and total trans-σ-HCA. Levels of bound trans-σ-HCA and free and bound cis-σ-HCA are calculated by appropriate subtraction.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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