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This article in CS

  1. Vol. 11 No. 2, p. 200-203
    Received: June 6, 1970

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Genetic Male Sterility in Barley. III. Pollen and Anther Characteristics1

  1. W. W. Roath and
  2. E. A. Hockett2



Pollen staining, pollen and anther appearance, and anther size were observed in 18 genetic male sterile mutants in barley, Hordeum vulgare L. The stains used were 2,3,5-triphenyltetrazolium chloride (TTC), acetocarmine, and iodine potassium iodide. Stain uptake with the vital stain TTC was not a good indication of selfing ability, but nonstainability indicated sterility. To facilitate allelism studies four groups of mutants were delimited: A. Selfing ability-ms9; B. Near-normal pollen and anther development but no selfing-ms6, ms8, ms16; C. Extremely abnormal pollen and anther development- ms3, ms7; and D. Variable pollen and anther development-ms1, ms2, ms4, ms5, ms10, ms11, ms11az, ms13, ms14, ms17, ms18, ms19. The two allelic mutants, ms11 and ms11az, behaved differently for pollen stainability and appearance and for anther size.

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