About Us | Help Videos | Contact Us | Subscriptions



This article in CS

  1. Vol. 12 No. 2, p. 243-244
    Received: Aug 21, 1971

Request Permissions


Germination of Cotton (Gossypium hirsutum L.) Pollen on an Artificial Medium1

  1. Richard M. Taylor2



Gossypium hirsutum L. pollen was germinated by dusting it on a 3.5% agar medium containing sucrose, manganous sulfate, calcium nitrate, and boric acid. The medium was autoclaved for 5 min at 15 pounds per square inch and then poured into petri dishes to a depth of 5 to 7 mm. After cooling, the agar plates were stored in a refrigerator for 24 hours before use. Pollen was dusted onto the medium and the plates were placed in a germination chamber at 30 C and a relative humidity approaching 100%. To date, germinations have been low, ranging from 10 to 64% with an average of 30%. Advantages of this technique over the Broncker's method are: (i) more rapid germination (2 to 3 hours); (ii) more normal-appearing tube cytoplasm, and (iii) much longer tubes.

  Please view the pdf by using the Full Text (PDF) link under 'View' to the left.

Copyright © .