Germination of Cotton (Gossypium hirsutum L.) Pollen on an Artificial Medium1
- Richard M. Taylor2
Gossypium hirsutum L. pollen was germinated by dusting it on a 3.5% agar medium containing sucrose, manganous sulfate, calcium nitrate, and boric acid. The medium was autoclaved for 5 min at 15 pounds per square inch and then poured into petri dishes to a depth of 5 to 7 mm. After cooling, the agar plates were stored in a refrigerator for 24 hours before use. Pollen was dusted onto the medium and the plates were placed in a germination chamber at 30 C and a relative humidity approaching 100%. To date, germinations have been low, ranging from 10 to 64% with an average of 30%. Advantages of this technique over the Broncker's method are: (i) more rapid germination (2 to 3 hours); (ii) more normal-appearing tube cytoplasm, and (iii) much longer tubes.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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