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This article in CS

  1. Vol. 14 No. 6, p. 854-856
     
    Received: Apr 5, 1974


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doi:10.2135/cropsci1974.0011183X001400060024x

Analysis by Digestion and Colorimetric Assay of Total Nitrogen in Plant Tissues High in Nitrate1

  1. D. A. Cataldo,
  2. L. E. Schrader and
  3. V. L. Youngs2

Abstract

Abstract

A method is proposed for analysis of total nitrogen in plant tissues enabling predigestion (to reduce nitrate to ammonium) and digestion to be performed in a Folin-Wu tube. Following digestion, a sensitive colorimetric assay for ammonium is used to quantitate N-content in an aliquot of the digest. The proposed method has several advantages: 1) up to 110 tissue samples can be predigested and digested in one batch; 2) small tissue samples (5 to 100 mg) can be accommodated; 3) the colorimetric assay for ammonia is very sensitive; 4) tissues can be predigested to recover all nitrate in the samples; and 5) aliquot size from the diluted digest can be varied to effectively detect as little as 1 µg of N.

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