Analysis by Digestion and Colorimetric Assay of Total Nitrogen in Plant Tissues High in Nitrate1
- D. A. Cataldo,
- L. E. Schrader and
- V. L. Youngs2
A method is proposed for analysis of total nitrogen in plant tissues enabling predigestion (to reduce nitrate to ammonium) and digestion to be performed in a Folin-Wu tube. Following digestion, a sensitive colorimetric assay for ammonium is used to quantitate N-content in an aliquot of the digest. The proposed method has several advantages: 1) up to 110 tissue samples can be predigested and digested in one batch; 2) small tissue samples (5 to 100 mg) can be accommodated; 3) the colorimetric assay for ammonia is very sensitive; 4) tissues can be predigested to recover all nitrate in the samples; and 5) aliquot size from the diluted digest can be varied to effectively detect as little as 1 µg of N.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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