Plant Regeneration from Tissue Cultures of Maize1
- C. E. Green and
- R. L. Phillips2
Effective utilization of cell and tissue culture methods in Zea mays research requires cultures capable of plant regeneration. These differentiated plants would provide a direct link with conventional genetic and breeding procedures.
Maize callus from embryo scutellar tissues was initiated and maintained on MS medium inorganic components, Straus medium vitamins and amino acids, 20 g sucrose and 8 g agar per liter, and 2 mg 2,4-dichlorophenoxyacetic acid (2,4-D)/liter. Callus has been maintained subculture every 21 to 28 days and has remained capable of differentiation for 9 months. Regeneration of complete plants was accomplished by subculture of callus to 0.25 mg 2,4-D/liter for 30 days followed by transfer to 2,4-D-free culture medium. At 0.25 mg 2,4-D/liter numerous curled and wrinkled leaves developed. Approximately 200 complete plants have been differentiated. after transfer to the 2,4-D-free medium. Root tip cells from five plants indicated that each had 20 chromosomes. After transplantation to soil, 10 to 15% of the plants survived and grew normally. The optimum embryo age for scutellar callus initiation was 18 days post-pollination. Hormone combinations such as 1 mg 2,4-D, 4 mg a-naphthaleneacetic acid (NAA), and 0.05 mg 6-(γ,γ-dimethyl allylamino)-purine (2iP)/liter may increase the efficiency of scutellar callus initiation.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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