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This article in CS

  1. Vol. 15 No. 4, p. 550-553
    Received: Dec 5, 1974

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Studies on Male Sterility in Barley Induced by Sodium 1-(p-Chlorophenyl) 1,2-Dihydro-4,-6-Dimethyl-2-Oxonicotinate1

  1. R. C. Wang and
  2. Steve Lund2



Foliar sprays with various rates of an aqueous solution of sodium 1-(p-chlorophenyl) 1,2-dihydro-4,6-dimethyl-2- oxonicotinate (RH-531) were applied to barley (Hordeum vulgate L.) plants at different stages of growth to find the optimum application time and dosage for induction of complete male sterility. Under greenhouse conditions, 1.5 kg/ha a.i. of RH-531 applied several days before meiosis in pollen mother cells (about six.leaf stage or 20 days before heading) induced complete male sterility. At least 19% female fertility was present on RH-531. induced male-sterile ‘Barsoy’ plants, estimated by natural pollination. Female fertility of RH-531.induced malesterile ‘Larker’ barley was 40% of the control when both were hand pollinated.

Meiosis in pollen mother cells did not appear to be affected by RH-531. Paraffin sections of anthers from RH-531-induced lnale-sterile barley revealed abnormal persistence of the tapetum. Acetocarmine was inferior to iodine-potassium iodide (IKI) and Alexander's multiple stain for determining pollen viability in RH-531- treated barley plants. Staining of pollen grains with IKI suggested that pollen sterility induced by RH-531 was related to an absence of or a decrease in carbohydrates.

Positive correlations were found between percentage seed-set of bagged spikes and anther length, and between percentage seed-set of bagged spikes and pollen grain carbohydrate content. It appeared that RH-531 inhibited anther growth thus leading to either degeneration of microspores or reduction in carbohydrates in pollen grains.

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