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This article in CS

  1. Vol. 15 No. 6, p. 750-752
     
    Received: Jan 9, 1975


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doi:10.2135/cropsci1975.0011183X001500060003x

Influence of Osmotic Potential on the Growth and Development of Soybean Tissue Cultures1

  1. S. L. Kimball,
  2. W. D. Beversdorf and
  3. E. T. Bingham2

Abstract

Abstract

The influence of reduced osmotic potentials on soybean (Glycine max L.) tissue cultures was evaluated. Reduced osmotic potentials were generated by addition of mannitol, sorbitol, glucose, or sucrose to a standard medium. Cells in control callus were comparatively large and about half were elongated or irregularly shaped. As osmotic potential decreased, all cells became smaller and spherical, regardless of which osmoticum was used. The addition of metabolically active or inert osmotica resulted in increased callus production. Maximum callus growth (fresh weight) occurred when the osmotic potential of the culture medium was lowered 8 to 12 bars. Decreasing osmotic potentials by 2 to 20 bars did not increase the frequency of adventitious bud differentiation above the low frequency observed on the standard medium.

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