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This article in CS

  1. Vol. 15 No. 6, p. 853-855
     
    Received: May 22, 1975


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doi:10.2135/cropsci1975.0011183X001500060031x

Laboratory Technique for Bleaching Pea Seeds1

  1. W. G. Riehle and
  2. F. J. Muelbauer2

Abstract

Abstract

Conditions for bleaching pea (Pisum sativum L.) seeds in the laboratory were studied to find a method of identifying bleach-resistant selections in the early stages of a breeding program. Among the genotypes used, differential seed bleaching without germination was obtained when 4.2 g pea seed (about 25 seeds) was placed on five sheets of Whatman no. 1 filter paper in 9-cm petri dishes and moistened with 6 ml water. The petri dishes were then placed in a growth chamber at 23 C with 8.6 klux of continuous light. After 8 days, the seed coats were removed, and the cotyledons were scored on a scale from 0 for no bleaching to 4 for complete bleaching. The technique was highly correlated (r = 0.78**, df = 27) with seed bleaching occurring in the field and should therefore be useful for early generation testing of green, dry-edible pea selections.

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