About Us | Help Videos | Contact Us | Subscriptions



This article in CS

  1. Vol. 15 No. 6, p. 853-855
    Received: May 22, 1975

Request Permissions


Laboratory Technique for Bleaching Pea Seeds1

  1. W. G. Riehle and
  2. F. J. Muelbauer2



Conditions for bleaching pea (Pisum sativum L.) seeds in the laboratory were studied to find a method of identifying bleach-resistant selections in the early stages of a breeding program. Among the genotypes used, differential seed bleaching without germination was obtained when 4.2 g pea seed (about 25 seeds) was placed on five sheets of Whatman no. 1 filter paper in 9-cm petri dishes and moistened with 6 ml water. The petri dishes were then placed in a growth chamber at 23 C with 8.6 klux of continuous light. After 8 days, the seed coats were removed, and the cotyledons were scored on a scale from 0 for no bleaching to 4 for complete bleaching. The technique was highly correlated (r = 0.78**, df = 27) with seed bleaching occurring in the field and should therefore be useful for early generation testing of green, dry-edible pea selections.

  Please view the pdf by using the Full Text (PDF) link under 'View' to the left.

Copyright © .