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This article in CS

  1. Vol. 17 No. 5, p. 811-813
    Received: Dec 13, 1976

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Inheritance of a Second Trypsin Inhibitor Variant in Seed Protein of Soybeans1

  1. J. H. Orf and
  2. T. Hymowitz2



Soybean [Glycine max (L.) Merrill] seeds from selfed heterozygous F2 plants of the cross ‘Harosoy’ ✕ P-I- 246367 and F2 seeds from reciprocal crosses of T245 and P.I. 246367 were analyzed for electrophoretic banding patterns of SBTI-A2 (Kunitz inhibitor). Harosoy seed has the SBTI-A2 band at Rf 0.79 (Ti1), T245 at Rf 0.75 (Ti3) and P.I. 246367 at Rf 0.83. Seeds having two bands produced plants in which seeds segregated in a 1:2:1 ratio whether in the F2, F3, or F4 generation. All seed with only one band bred true in the next generation. These data along with data previously reported establishes that three forms of the SBTI-A2 designated as Ti1, Ti2, and Ti3 are electrophoretically distinguishable from one another by their different Rf values of 0.79, 0.75, and 0.83, respectively. The forms are controlled by a codominant multiple allelic system at a single locus

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