Root and Shoot Development from Sugarcane Callus Tissue1
- H. M. Nadar and
- D. J. Heinz2
Growth of shoots differentiated from sugarcane (Saccharum sp.) callus was enhanced by 5 mg naphthalene acetic acid (NAA)/I in the absence of coconut water, while 0.2 mg 2,4-dichlorophenoxyacetic acid (2,4-D)/1 stunted their growth. In the presence of NAA, a crown developed at the base of the shoot on which new shoots and roots developed, grew, and formed a clump of plantlets.
Root growth could also be induced by trimming the leaves or by incubation at 15C for 4 to 6 weeks. Leaf trimming resulted in more vigorous root growth than cold treatment, although clonal differences were observed.
Results indicate that a medium which stimulates differentiation does not necessarily stimulate shoot growth, and the hormonal requirements for shoot differentiation differ from requirements for root induction in sugarcane callus.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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