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This article in CS

  1. Vol. 17 No. 5, p. 814-816
    Received: Dec 9, 1976

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Root and Shoot Development from Sugarcane Callus Tissue1

  1. H. M. Nadar and
  2. D. J. Heinz2



Growth of shoots differentiated from sugarcane (Saccharum sp.) callus was enhanced by 5 mg naphthalene acetic acid (NAA)/I in the absence of coconut water, while 0.2 mg 2,4-dichlorophenoxyacetic acid (2,4-D)/1 stunted their growth. In the presence of NAA, a crown developed at the base of the shoot on which new shoots and roots developed, grew, and formed a clump of plantlets.

Root growth could also be induced by trimming the leaves or by incubation at 15C for 4 to 6 weeks. Leaf trimming resulted in more vigorous root growth than cold treatment, although clonal differences were observed.

Results indicate that a medium which stimulates differentiation does not necessarily stimulate shoot growth, and the hormonal requirements for shoot differentiation differ from requirements for root induction in sugarcane callus.

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