Inheritance of the Absence of the Kunitz Trypsin Inhibitor in Seed Protein of Soybeans1
- J. H. Orf and
- T. Hymowitz2
Soybean [Glycine max (L.) Merrill] seds from reciprocal crosses of ‘Amsoy 71’ and P.I. 157440, reciprocal crosses of P.I. 196172 and P.I. 157440, and the cross P.I. 157440 ✕ ‘Jefferson’ were analyzed using polyacrylamide gel electrophoresis to study the inheritance of the absence of the Kunitz trypsin inhibitor (SBTI-A2). Amsoy 71 has the SBTI-A2, at Rf 0.79 (Tia), Jefferson at Rf 0.75 (Tib), P.I. 196172 at Rf 0.83 (Tic), and P.I. 157440 lacks the SBTI-A2, band. The F2 and F3 data establish that the absence of the SBTI-A2, band is inherited as a recessive to the codominant alleles Tia, Tib, and Tic. The allele for absence of the SBTI-A2 band is designated ti. The homozygous recessive condition (ti ti) results in the lack of SBTI-A2. The lack of the SBTI-A2, protein was confirmed using spectrophometric assay procedures.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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