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This article in CS

  1. Vol. 19 No. 1, p. 117-118
     
    Received: June 5, 1978


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doi:10.2135/cropsci1979.0011183X001900010029x

Regeneration of Plantlets from Callus Cultures of Indiangrass1

  1. C. H. Chen,
  2. P. F. Lo and
  3. J. G. Ross2

Abstract

Abstract

Plantlets were regenerated through organogenesis from the callus cultures of 10 agronomically superior genotypes of Indiangrass (Sorghastrum nutans L Nash). Callus was first initiated from segments of young inflorescences explanted on RM medium supplemented with hormonal combinations of 5 or 10 mg 2,4-D and 0 or 0.2 mg kinetin/liter. Plantlets were then induced from the calluses by subculturing on RM medium with kinetin omitted and 2,4-D reduced below 1 mg/liter. Of the media tested, callus grew best on RM medium supplemented with 5 mg 2,4-D and 0.2 mg kinetin/liter. Differential responses to the morphogenetic induction medium were noted among the 10 genotypes tested.

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