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Crop Science Abstract -

Regeneration of Plantlets from Callus Cultures of Indiangrass1


This article in CS

  1. Vol. 19 No. 1, p. 117-118
    Received: June 5, 1978

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  1. C. H. Chen,
  2. P. F. Lo and
  3. J. G. Ross2



Plantlets were regenerated through organogenesis from the callus cultures of 10 agronomically superior genotypes of Indiangrass (Sorghastrum nutans L Nash). Callus was first initiated from segments of young inflorescences explanted on RM medium supplemented with hormonal combinations of 5 or 10 mg 2,4-D and 0 or 0.2 mg kinetin/liter. Plantlets were then induced from the calluses by subculturing on RM medium with kinetin omitted and 2,4-D reduced below 1 mg/liter. Of the media tested, callus grew best on RM medium supplemented with 5 mg 2,4-D and 0.2 mg kinetin/liter. Differential responses to the morphogenetic induction medium were noted among the 10 genotypes tested.

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