Regeneration of Plantlets from Callus Cultures of Indiangrass1
- C. H. Chen,
- P. F. Lo and
- J. G. Ross2
Plantlets were regenerated through organogenesis from the callus cultures of 10 agronomically superior genotypes of Indiangrass (Sorghastrum nutans L Nash). Callus was first initiated from segments of young inflorescences explanted on RM medium supplemented with hormonal combinations of 5 or 10 mg 2,4-D and 0 or 0.2 mg kinetin/liter. Plantlets were then induced from the calluses by subculturing on RM medium with kinetin omitted and 2,4-D reduced below 1 mg/liter. Of the media tested, callus grew best on RM medium supplemented with 5 mg 2,4-D and 0.2 mg kinetin/liter. Differential responses to the morphogenetic induction medium were noted among the 10 genotypes tested.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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