Improved Rate of Callus and Green Plant Production from Rice Anther Culture Following Cold Shock1
- A. Dennis Genovesi and
- Clint W. Magill2
Rice (Oryza sativa L. subsp. japonica) panicles containing pollen at the G1 G2, early binucleate, and late binucleate stages of development were subjected to 5, 10, and 13 C temperatures for 3, 7, 10, 14, and 21 days before placing the anthers on callus-inducing medium. The uninucleate stages gave much higher frequencies of callus induction in both control and treated samples. In general, the more moderate temperature over a 10- to 14-day treatment gave maximum frequency of callus induction. While most of the plants derived from G1 and G2 stage anthers stored in the cold for 2 weeks or less were green, plants from later pollen stages or 21 days of cold storage were predominately albino.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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