Plant Regeneration from Callus Culture in Wheat1
- B. S. Ahloowalia2
Plant regeneration was investigated from embryo-callus culture in bread wheat, Triticum aestivurn L. Immature embryos of cultivar ‘Maris Huntsman’ were cultured on half-strength Murashlge and Skoog's medium (half-MS) supplemented with 0.5 mg 2,4-D[(2,4-dichlorophenoxy) acetic acid], 3.2 mg IAA (indole-3-acetic acid) and 1.0 mg kinetin [6-(furfurylamino) purlne]/liter or without kinetin. On medium with 2,4-D, IAA and kinetin, 77% of the embryos produced a visible callus within 2 weeks. On medium without kinetin, all embryos produced a callus. Calll were cloned and maintained for over 900 days by subculturing at 50 to 60 days interval on half-MS medium with 0.5 mg 2,4-D/llter but regeneration ceased after 680 days. Calli showed organogenesis within 120 days of subculture on half-MS medium with 0.5 mg 2,4-D and 1.0 mg zeatin [6-(4-hydroxy-3-methyl but-2 enylamlno) amino purine]/liter. Plantlets originated from callus by differentiation into shoot-primordia and bipolar structures. Plantlets produced roots on half-MS medium without growth regulators; however, 1.0 mg naphthalene acetic acld/llter in the medium promoted rooting. Regenerated plants included variants for plant height, stem thickness, leaf size, spike shape, pollen fertility and seed set.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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