Stable Cytoplasmic Male-Sterile Mutants Induced in Tift 23DB1 Pearl Millet with Mitomycin and Streptomycin 1
- Glenn W. Burton and
- W. W. Hanna2
Stable cytoplasmic male-sterile lines are necessary for the commercial production of F1 hybrid seed of important crop plants such as sorghum (Sorghum bicolor L.) and pearl millet [Pennisetum americanum (L.) Leeke.]. The objective of this investigation was to ascertain if streptomycin and mitomycin could help to create stable cms mutants. Seeds of ‘Tilt 23DB1’ pearl millet were soaked in water solutions of 200 and 500 ppm of streptomycin, and 50 ppm of mitomycin at 5 C for 40 hours. Treated seeds were rinsed in tap water, surface dried, and planted in an isolated field along with a check of untreated seed. Head populations of 32,800, 22,300, 18,000, and 13,000 from seed treated with 0, 200, 500 ppm of streptomycin and 50 ppm of mitomycin contained progeny tested stable cytoplasmic male-sterile (cms) mutants at frequencies of 1 in 16,200, 5,575, 4,500, and 2,600, respectively. The sterility or fertility of ctns mutants crossed with 2 sterility maintainers and 6 fertility restorer inbreds of pearl millet suggests that the induced mutants have sterility maintainer and fertility restorer requirements similar to cms Tilt 23DA, and are similar cms mutants.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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