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This article in CS

  1. Vol. 22 No. 3, p. 669-671
    Received: June 30, 1980

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Non-destructive Estimation of 14C in Soybeans Immediately After Labelling1

  1. J. R. Pearen and
  2. D. J. Hume2



Accurate measurement of 14C initially taken up in photosynthesis would allow estimation of respiratory losses from disappearances of 14C between labelling and subsequent harvests. Also, distribution of the photo-assimilates could be determined. This experiment was conducted to evaluate a nondestructive technique for estimating 14C in soybeans [Glycine max (L.) Merrill] immediately after labelling. Plants were grown in the field in buckets with nutrient solutions containing low N, NO3 - or NH4*. Leaflet areas were measured prior to labelling with 14CO2 at five different growth stages. Leaf discs removed immediately after labelling and leaf areas were used to estimate how much 14C was in each plant. In plants harvested immediately, the technique accounted for 81 to 97% of the variability in 14C contents at the five growth stages with the poorest precision occurring during seed-filling. The leaf disc technique allowed estimation of initial 14C in other plants left for later harvests.

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