The Effect of Rhizobium trifolii Strains and Crimson Clover Genotypes on N2 Fixation1
- G. R. Smith,
- W. E. Knight,
- H. L. Peterson and
- C. Hagedorn2
The effects of crimson clover (Trifolium incarnatum L.) genotype and strain of Rhizobium trifolii on dry weight yield and N2 fixation were investigated in a factorial experiment. The treatments were arranged in a randomized complete block design with eight replications. The clover-Rhizobium combinations were grown in plastic pouches with a N-free nutrient solution. A growth chamber was used to maintain 24/19 C diurnal temperatures and a 12 hour photoperiod.
After 60 days of growth, the combinations were assayed for N2 fixation using acetylene reduction and the plant tops were harvested and dried. Plant lines, strains of Rhizobium and line ✕ strain interaction were all significant sources of variation for both acetylene reduction rate and dry weight yield. Strains had the largest effect, in terms of variance components, on dry weight yield followed by plant lines and strain ✕ line interaction, respectively. Strains also had the largest effect on acetylene reduction rate followed by strain ✕ line interaction and plant lines, respectively. Strain TA1 of R. trifolii was highly variable in its effect on the different plant lines. Response of different inbred lines with this strain ranged from efficient to inefficient.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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