Techniques of Screening Peas for Resistance to Phoma medicaginis var. pinodella1
- D. Sakar,
- F. J. Muehlbauer and
- J. M. Kraft2
Footrot and foliar phases of the disease caused by Phoma medicaginis var. pinodella in peas (Pisum sativum L.) were studied. Major objectives were to develop repeatable inoculation procedures and to identify pea lines resistant to footrot and foliar infection. All tests were conducted under controlled environmental conditions. Fungal spore concentrations and plant age at inoculation were important for identifying differences in disease reactions among lines. Significant differences in reactions to footrot were obtained among lines when pea seeds were immersed for 5 min. in inoculum adjusted to 1 ✕ 104 spores/ml, planted in a sterile soil mixture, and scored 10 days later. Significant differences in resistance among pea lines to the foliar phase of the disease were obtained when 15-day-old pea seedlings were sprayed with inoculum containing 1 ✕ 105 spores/ml and scored 10 days later. Footrot scores ranged from 1.2 to 4.8, and foliar disease scores ranged from 2.1 to 4.9 when 96 pea genotypes were rated on a 1 to 5 scale (1 = symptomless and 5 = very severe disease). There was no significant correlation between footrot and foliar disease scores, indicating that different genetic factors may control reactions to the two phases of the disease.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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