Variation in the Low Molecular Weight Proteinase Inhibitors of Soybeans1
- R. W. Stahlhut and
- T. Hymowitz2
Two types of proteinase inhibitors exist in soybean [Glycine max (L.) Merr.] seed. The first type is called the Kunitz trypsin inhibitor and the second is a group generally termed the Bowman-Birk type inhibitors. The objectives of this study were to attempt to separate and purify the Bowman-Birk type inhibitors in soybean seeds, and to screen for variants in the USDA Named Cultivar and Type Collections. Five Bowman-Birk type inhibitors were separated from ‘Amsoy 71’ by anion exchange column chromatography. Soybean accessions were screened electrophoretically for variants of the inhibitors. Three variants were identified: 1) lines which appear to be missing fraction III electrophoretic band (F phenotype); 2) lines which appear to be missing fraction V electrophoretic band (S phenotype); and 3) lines in which both electrophoretic bands are present (SF phenotype). Most lines examined (71%) have the SF banding pattern. The S or F banding patterns are less common with only 26 and 3%, respectively, of the lines screened.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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