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Crop Science Abstract -

Response of Six Rice Callus Cultures to Deep-Frozen Temperatures1,3


This article in CS

  1. Vol. 24 No. 1, p. 82-85
    Received: June 11, 1982

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  1. J. M. Ulrich,
  2. B. J. Finkle,
  3. B. E. Mackey,
  4. G. W. Schaeffer and
  5. Frank Sharpe Jr.2



Callus cultures of five mutants of rice (Oryza sativa L.), which could grow in the presence of the synthetic analog of lysine, S-aminoethyl-L-cysteine, and the nonmutant strain from which the mutants were derived were studied for growth response after freezing at several temperatures. Although the mutant lines were derived from a single line, variations were noted in response to the mixture of cryoprotectants added [10% polyethylene glycol, 8% glucose, 10% dimethylsulfoxide (PGD) in H2O, w/v] and freezing treatments. Following freezing (and subsequent thawing), growth of callus was less at lower freezing temperatures; but all cultures grew even after freezing at —196°C. After the —196°C treatment, three lines increased three to four times the initial inoculum weight after 6-, 8-, and 10-week growth periods, compared to an increase of 14 times in 4 weeks for the cell line which grew most rapidly after thawing. Because all rice lines survived a brief period at —196°C and two lines tested, later survived —196°C for more than a year, the possibility exists for long-term, deep-frozen preservation of rice callus cultures.

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