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This article in CS

  1. Vol. 24 No. 1, p. 82-85
     
    Received: June 11, 1982


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doi:10.2135/cropsci1984.0011183X002400010018x

Response of Six Rice Callus Cultures to Deep-Frozen Temperatures1,3

  1. J. M. Ulrich,
  2. B. J. Finkle,
  3. B. E. Mackey,
  4. G. W. Schaeffer and
  5. Frank Sharpe2

Abstract

Abstract

Callus cultures of five mutants of rice (Oryza sativa L.), which could grow in the presence of the synthetic analog of lysine, S-aminoethyl-L-cysteine, and the nonmutant strain from which the mutants were derived were studied for growth response after freezing at several temperatures. Although the mutant lines were derived from a single line, variations were noted in response to the mixture of cryoprotectants added [10% polyethylene glycol, 8% glucose, 10% dimethylsulfoxide (PGD) in H2O, w/v] and freezing treatments. Following freezing (and subsequent thawing), growth of callus was less at lower freezing temperatures; but all cultures grew even after freezing at —196°C. After the —196°C treatment, three lines increased three to four times the initial inoculum weight after 6-, 8-, and 10-week growth periods, compared to an increase of 14 times in 4 weeks for the cell line which grew most rapidly after thawing. Because all rice lines survived a brief period at —196°C and two lines tested, later survived —196°C for more than a year, the possibility exists for long-term, deep-frozen preservation of rice callus cultures.

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