Identification of Cultivars of Perennial Ryegrass by SDS-PAGE of Seed Proteins1
- J. M. Ferguson and
- D. F. Grabe2
The number of cultivars of perennial ryegrass (Lolium perenne L.) continues to grow. Accurate and rapid laboratory techniques to differentiate between cultivars has become increasingly important for both consumer protection and plant variety protection. The purpose of our work was to develop an electorphoretic procedure for ryegrass seed which would differentiate cultivars of perennial ryegrass. Proteins were extracted from ground seeds of 28 perennial ryegrass cultivars. Extracts were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE) procedures. A total of 27 bands were present with many of the bands common for the 28 cultivars. Cultivars were characterized by presence or absence of specific bands and by band intensity ratios calculated from densitometer scans. The cultivars Pennant and Premier were not successfully differentiated from each other nor was ‘Omega’ found to be different from the cultivar Birdie. All other cultivars were differentiated by unique banding patterns. Banding patterns from SDS-PAGE of proteins were not affected by year and location of production, class of certified seed, or viability and vigor of the seed.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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