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This article in CS

  1. Vol. 26 No. 1, p. 189-191
     
    Received: Feb 19, 1985


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doi:10.2135/cropsci1986.0011183X002600010045x

Production of Freeze Inhibitor Polysaccharides of Rye and Barley in Tissue Culture1

  1. C. R. Olien,
  2. M. N. Smith and
  3. Paul K. Kindel2

Abstract

Abstract

Tissue cultures of Secale cereale L., (‘Rosen’ rye) and Hordeum vulgare (‘Hudson’ barley) were developed in order to produce larger quantities of freeze-inhibitor polysaccharidesle ss expensively than can presently be done from cereal crown tissue. Culture techniques were perfected so that viable aseptic callus was obtained from rye and barley seed embryo with greater than 95% success. The callus was grown on a solid medium for approximately 4 weeks at 23°Ca nd then transferred to liquid shake culture, first at 23°C and then at 0°C. The freeze-inhibitor ratings of polysaccharides produced by rye and barley in tissue culture were similar to those of polysaccharides extracted from hardened crown tissues of the same cultivars. Arabinose and xylose constituted greater than 75% of the unpurified freezeinhibitor polysaccharidesp roducedin culture.

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