Plant Regeneration in Callus Cultures Derived from Young Inflorescences of Little Bluestem1
- D. D. Songstad,
- C. H. Chen and
- A. A. Boe2
Little Bluestem [Schizachyrium scoparium (Michx.) Nash], a warm-season perennial grass, is valuable for range and conservation plantings in the Great Plains. The objectives of this study were to develop rapid vegetative propagation techniques to facilitate selection of superior genotypes and to elucidate differentiation patterns in tissue culture. Embryogenic call! were induced from 5- to 10-mm segments of unemerged young inflorescences and maintained on Linsmaier and Skoog's RM medium supplemented with 5 mg 2,4-Dichlorophenoxy acetic acid (2,4-D)/L in the dark at 25°C. The explants were furnished from three genotypes selected from a breeding nursery. Plants regenerated through somatic embryogenesis from callus were grown on RM medium containing no hormones and with the same physical conditions as for callus initiation. Embryogenesis tended to be suppressed by 2,4-D concentrations of 1 mg/L or higher, but was promoted by the addition of 1 to 5 mg kinetin/L. Regenerated plants grew to maturity in 24 weeks after being transplanted into pots in the greenhouse and were phenotypically similar to their source plants.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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