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This article in CS

  1. Vol. 27 No. 4, p. 806-810
     
    Received: Aug 13, 1986


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doi:10.2135/cropsci1987.0011183X002700040041x

A Hybrid Seed Production Method Based on Synthesis of Novel Linkages between Marker and Male-Sterile Genes1

  1. Richard A. Jorgensen2

Abstract

Abstract

Mendelian male-sterile genes are rarely used in hybrid seed production because pure stands of male-sterile plants are difficult to obtain. We describe here a general method for hybrid seed production whereby linkage is synthesized between a male-sterile gene and a marker gene through genetic transformation. Such linkage permits the production of nearly pure stands of male-sterile plants. The method should be applicable in any plant species in which markers can be introduced into approximately random genomic locations. It is shown that the Agrobacterium transformation system can be used to synthesize tight, novel linkages between markers and male-sterile genes in tomato (Lycopersicon esculentum Mill.). In addition, a marker gene is identified that is potentially suitable for use in the proposed hybrid production system in several crops. This marker, IaaH, determines a dominant chemical sensitivity phenotype which, if linked in coupling phase with a male-sterile gene, should allow chemical removal of fertiles from a population segregating for male sterility/fertility and chemical resistance/sensitivity.

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