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This article in CS

  1. Vol. 27 No. 5, p. 869-872
     
    Received: Nov 7, 1986


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doi:10.2135/cropsci1987.0011183X002700050007x

Inheritance of Aflatoxin B1 Levels in Maize Kernels under Modified Natural Inoculation with Aspergillus flavus1

  1. L. L. Darrah,
  2. E. B. Lillehoj,
  3. M. S. Zuber,
  4. G. E. Scott,
  5. D. Thompson,
  6. D. R. West,
  7. N. W. Widstrom and
  8. B. A. Fortnum2

Abstract

Abstract

Several earlier studies on aflatoxin contamination in maize (Zea mays L.) have shown significant differences among genotypes, but the results have been difficult to repeat, probably due in part to a very large environment-by-genotype interaction resulting in large coefficients of variation. A second problem encountered has been lack of a suitable method of inoculation. A genetic study at the University of Missouri published in 1978 employing the pinboard method of inoculation showed significant differences among genotypes, and these results using the same genotypes were substantiated, in part, by a second study. These and later studies, however, did not provide comparative information on aflatoxin contamination by pinboard inoculation with Aspergillus flavus (Link ex Fries) and contamination in the absence of wounding of kernels. Twenty-one F1 crosses of seven inbred lines of maize that had been used in two previous studies plus the seven parents, were planted in five environments in the southeastern USA where the likelihood of natural contamination by A. flavus would be high. Natural contamination was enhanced by spraying conidia of A. flavus on the silks at each environment. Aflatoxin contamination was observed to be highly significantly different among genotypes and environments. Mean aflatoxin B1 levels ranged from a low of 54 μg kg−1 at Knoxville, TN, to 1788 μg kg−1 at Mississippi State, MS. The general combining ability mean square for aflatoxin B1 was significant. Comparison of relative aflatoxin levels by inbred line means, F1 crosses per se, or estimates of general combining ability effects obtained from the current study with results of pinboard inoculation showed negative relationships. The results showed that information on geneticontrol of aflatoxin contamination in maize obtained by kernel wounding using a pinboard and inoculation with A. flavus was not the same as that obtained in the absence of kernel wounding.

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Copyright © 1987. Crop Science Society of America, Inc.Copyright © 1987 by the Crop Science Society of America, Inc.