About Us | Help Videos | Contact Us | Subscriptions
 

Abstract

 

This article in CS

  1. Vol. 30 No. 1, p. 226-229
     
    Received: Mar 13, 1989
    Published: Jan, 1990


    * Corresponding author(s):
 View
 Download
 Alerts
 Permissions
 Share

doi:10.2135/cropsci1990.0011183X003000010049x

Evaluation of a New In Vitro Cell Selection Technique

  1. Maria Tomaso-Peterson  and
  2. J. V. Krans
  1. Dep. of Agronomy, Mississippi State Univ., P.O. Box 5248, Mississippi State, MS 39762

Abstract

Abstract

In vitro cell selection is a novel means of recovering elite plant types. This study evaluated a new in vitro cell selection technique [Host-Pathogen Interaction System (HPIS)] that exposes call! to a pathogen without physical contact between the organisms. The material components that make up the HPIS are a double-sided petri dish (Lutri-plate®), a polycarbonate Nucleopore® membrane, caulking cord, and the appropriate agar media. Callus of ‘Penncross’ creeping bentgrass (Agrostis palustris Huds.) and two isolates of Rhizoctonia solani Kuhn. (RVPI and RATCC) were used in evaluating the HPIS. After 12 d of coculture in the HPIS, callus fresh weights were less in plates cocuitured with RVPI (−0.09 g) and RATC(C0 .47 g) isolates than control plates (0.95 g). Callus cocuitured in the HPIS with either isolate displayed no plantlet formation, while control callus cultured in the HPIS without the fungi formed numerous vigorous plantlets. The HPIS appears to provide an effective means for exposing calli to a pathogen without the complications associated with direct physical contact between the organisms. Research is currently underway to utilize the HPIS for recovering desirable variants of Penncross with resistance to R. solani.

Contribution from the Mississippi Agric. and Forestry Exp. Stn. Assigned journal no. J-7145.

  Please view the pdf by using the Full Text (PDF) link under 'View' to the left.

Copyright © 1990. Crop Science Society of America, Inc.Copyright © 1990 by the Crop Science Society of America, Inc.