Evaluation of a New In Vitro Cell Selection Technique
- Maria Tomaso-Peterson and
- J. V. Krans
In vitro cell selection is a novel means of recovering elite plant types. This study evaluated a new in vitro cell selection technique [Host-Pathogen Interaction System (HPIS)] that exposes call! to a pathogen without physical contact between the organisms. The material components that make up the HPIS are a double-sided petri dish (Lutri-plate®), a polycarbonate Nucleopore® membrane, caulking cord, and the appropriate agar media. Callus of ‘Penncross’ creeping bentgrass (Agrostis palustris Huds.) and two isolates of Rhizoctonia solani Kuhn. (RVPI and RATCC) were used in evaluating the HPIS. After 12 d of coculture in the HPIS, callus fresh weights were less in plates cocuitured with RVPI (−0.09 g) and RATC(C0 .47 g) isolates than control plates (0.95 g). Callus cocuitured in the HPIS with either isolate displayed no plantlet formation, while control callus cultured in the HPIS without the fungi formed numerous vigorous plantlets. The HPIS appears to provide an effective means for exposing calli to a pathogen without the complications associated with direct physical contact between the organisms. Research is currently underway to utilize the HPIS for recovering desirable variants of Penncross with resistance to R. solani.
Copyright © 1990.