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This article in CS

  1. Vol. 30 No. 4, p. 871-874
     
    Received: Oct 16, 1989


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doi:10.2135/cropsci1990.0011183X003000040021x

Backcross-Derived Progeny from Soybean and Glycine tomentella Hayata Intersubgeneric Hybrids

  1. R. J. Singh,
  2. K. P. Kollipara and
  3. T. Hymowitz 
  1. Dep. of Agronomy, Univ. of Illinois, Urbana, IL 61801

Abstract

Abstract

Wild perennial species of the subgenus Glycine Willd, have not been exploited in soybean [Glycine max (L.) Merr.] breeding programs. This paper discusses wide hybridization in the genus Glycine and reports on the origin, identification, and breeding behavior of a synthesized amphiploid soybean ✕ G. tomentella Hayata hybrid and its backcross-derived progeny. Amphiploid H213-2a (2n = 118) plants showed almost normal meiotic pairing (0.711 + 57.9811 + 0.33IV), but later meiotic stages were abnormal, showing laggards and bridges, and the plants set only a few pods. The F2, F3, and F4 plants carried the expected 2n = 118 chromosomes, and thus were cytologically stable. A growth-hormone solution containing 100 mg gibberellic acid (GA3), 25 mg naphthalene acetic acid (NAA) and 5 mg kinetin/L distilled water was sprayed onto the pollinated gynoecia to enhance pod retention (14.19%) in H213-2a ✕ soybean crosses. Without daily hormone spray, only three pods of the 3417 florets pollinated were harvested 19 to 21 d after pollination. The BC1 plants were obtained in two cross combinations: (i) H213-2a ✕ ‘Clark 63’ = H562, and (ii) H213-2a ✕ ‘Essex’ = H564. The H562 plants contained 2n = 76, (BC1; expected 2n = 79), suggesting the loss of three chromosomes. The H564 plants contained 2n = 98 chromosomes and may have originated by the union of a female gamete (n = 59) and an unreduced male (n = 40) spore. All the BC1 plants were totally sterile, and attempts are being made to generate BC2 plants.

Research supported in part by the Illinois Agric. Exp. Stn and by USDA Competitive Research Grant 88-37231-4100.

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