Pollen Fertility of Some Simple and Compound Translocations of Cotton
- David M. Stelly ,
- Kenneth C. Kautz and
- William L. Rooney
Pollen semisterility can serve as a useful criterion for detecting cytogenetic deficiencies and heterozygosity of cytogenetic aberrations in many diploid crops. A modified form of the fluorescein diacetate-based fluorochrome reaction method was recently developed and found to allow reliable detection of cytogenetically induced pollen semisterility of cotton (Gossypium hirsutum L., 2n = 4x = 52) simple reciprocal translocation heterozygotes. We report the pollen fertility levels of plants either homozygous (TT) or heterozygous (NT) for 20 translocations, including 16 two-chromosome, three three-chromosome, and one four-chromosome translocations. Fertility ranged from 90 to nearly 100% for TT and from 52 to 81% for NT plants, indicating that TT and NT cytogenetic types in testcross populations that are segregating for a single translocation should be distinguishable on the basis of pollen fertility. Thus, all 45 breakpoints of the screened translocations are of potential use as genetic markers. These and previously published results demonstrate collectively that all identified translocations of G. hirsutum are amenable to pollen analysis by fluorescence microscopy, even though the species is a disomic tetraploid.
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