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This article in CS

  1. Vol. 30 No. 6, p. 1343-1350
    Received: Dec 4, 1989

    * Corresponding author(s):
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Expression of Maturation-Specific Genes in Soybean Seeds

  1. Yue-ie C. Hsing,
  2. Robert W. Rinne ,
  3. Angus G. Hepburn and
  4. Raymond E. Zielinski
  1. I nst. of Botany, Academia Sinica, Taipei, Taiwan
    P lant Physiology and Genetics Res. Unit, USDA-ARS, and Dep. of Agronomy
    D ep. of Agronomy, Univ. of Illinois, 1102 S. Goodwin, Urbana, IL 61801
    D ep. of Plant Biology, Univ. of Illinois, 505 S. Goodwin, Urbana, IL 61801



This laboratory, has previously shown (Rosenberg and Rinne, 1988, Plant Physiol. 87:474-478) that several polypeptides are synthesized de novo during natural and precocious soybean [Glycine max (L.) Merr.] seed maturation. The present study was undertaken to investigate soybean seed maturation at the molecular level. The objective of this investigation was to see if changes could be observed at the molecular level that support our previous physiological and biochemical studies. A cDNA library was prepared from poly A* RNA from precociously dried soybean seeds and screened by differential hybridization with probes made from poly A* RNAs isolated from fresh and pod-dried seeds to isolate dehydration-related cDNA clones. Hybrid-selected translation, cross-hybridization, and DNA sequencing experiments using five clones with the strongest hybridization signals revealed that each appears to encode a 31-kD polypeptide and represent the same mRNA sequence. The mRNA encoding this protein is expressed exclusively in maturing seeds from =55 d after flowering through maturity. Expression of the 31- kD polypeptide mRNA increases about 200-fold during soybean seed maturation in situ, and it can be induced to a comparable level of expression by precociously maturing 35 d after flowering seeds. Expression of this mRNA occurs predominantly after expression of the genes encoding the major storage proteins, and it coincides temporall with seed desiccation.

Part of a dissertation submitted by Y.C. Hsing to the Univ. of Illinois in partial fulfillment of the Ph.D. degree. This paper reports results of research only and the research was supported in part by the Univ. of Illinois College of Agriculture Fellowship awarded to Y.C.H. and in part by grants Hatch 15-363 awarded to A.G.H. and USDA-CRGO 86 AGCRCR 11932 awarded to R.E.Z.

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