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This article in CS

  1. Vol. 31 No. 2, p. 475-476
     
    Received: May 1, 1990
    Published: Mar, 1991


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doi:10.2135/cropsci1991.0011183X003100020050x

Cross-Pollination Technique for Spontaneously Self-Pollinated Sweetclover

  1. J. E. Miller,
  2. D. R. Viands ,
  3. T. A. LaRue and
  4. H. J. Gorz
  1. Dep. of Plant Breeding and Biometry, 252 Emerson Hall, Cornell Univ., Ithaca, NY 14853-1902

Abstract

Abstract

There is increased interest in using a dwarf, spontaneously self-pollinated accession (U389) of annual, white-flowered sweetclover (Melilotus alba Desr.) for studying N2 fixation and for characterizing the photosynthetic apparatus. Genetic analysis of this accession may therefore be required. In a study of the inheritance of 10 nonnodulating U389 mutants > 2000 crosses made in the late bud stage were unsuccessful probably because florets were mishandled during the crossing procedure. Development of an improved crossing technique resulted in production of hybrid seed from 20% of the emasculated and pollinated florets. This successful technique is described in detail for future use by researchers making crosses with spontaneously self-pollinated sweetclovers similar to U389.

Contribution of the Dep of Plant Breeding and Biometry New York State College of Agric. and Life Sci., Cornell Univ., and Boyce Thompson Inst., Ithaca, NY. Paper no. 795.

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