Regeneration of Peanut and Perennial Peanut from Cultured Leaf Tissue
- A. H. McKently ,
- G. A. Moore and
- F. P. Gardner
Successful utilization of biotechnology for the improvement of peanut, Arachis hypogaea L., will be made possible through development and optimization of tissue culture regeneration systems. This study evaluated plant development via organogenesis from in vitro-cultured immature leaf tissue of the cultivated peanut and a perennial peanut species, A. glabrata Benth. Leaflets, 5 mm in length from peanut seedlings and 5 to 10 mm in length from perennial peanut plants, were cultured in vitro on Murashige and Skoog medium supplemented with 1 mg L−1 1-naphthaleneacetic acid (NAA) and four concentrations (1, 3, 5, and 10 mg L−1) of 6-benzylaminopurine ( BA). Bud regeneration occurred from the adaxial surface of the cultivated peanut explants on all BA concentrations, with the largest quantity produced on 5 mg L−1. Eighty-four percent of the cultures with bud tissue continued growth and differentiation into shoots within 120 d. These shoots developed roots within 30 d of transfer to basal medium supplemented with 1 mg L−1 NAA. Plantlets transferred to soil and placed in a greenhouse developed successfully, matured, and set seed. Phenotypic variation was not observed. A wide range of cultivated peanut genotypes was evaluated for organogenic responsiveness. The perennial peanut leaf explants callused and produced shoot meristems within 50 d of culture. Ten percent of the meristems continued growth and development into whole plants.
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