Tissue Culture Regeneration of Desmodium
- D. S. Wofford ,
- K. H. Quesenberry and
- D. D. Baltensperger
Efficient tissue culture techniques for plant regeneration are known for many crops, but very considerably among species. These procedures need to be investigated, however, in crops that have no known method of regeneration. Six genotypes of two species of Desmodium were evaluated for tissue culture response on two protocols, an L2 basal salts + growth regulators embryogenic protocol and an MS basal salts + growth regulators organogenic protocol. Significantly greater callus growth was observed on the L2 callus induction medium for four of the six genotypes, with an average of 476.6 mg callus−1 for all genotypes on L2 compared with 47.9 mg callus−1 for the MS treatment. Based on L2 callus fresh weight data, genotypes could be classified into two groups, good and poor callus producers. Despite the overall poor callus growth on MS, shoot buds were induced for five of the six genotypes on the MS shoot induction medium. No genotypes produced somatic embryos on the L2 protocol; however, two genotypes, CIAT 6123 and CIAT 13083, did exhibit organogenesis on the L2 protocol. Plants were regenerated from CIAT 6123 on the L2 system. No shoot development was observed for any genotype on the MS treatment. Regenerated plants were phenotypically normal and no somaclonal variation was observed. Media modifications will be necessary in order to successfully manipulate Desmodium genotypes in vitro.
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