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This article in CS

  1. Vol. 32 No. 2, p. 377-383
     
    Received: Mar 4, 1991


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doi:10.2135/cropsci1992.0011183X003200020020x

Mapping Phytophthora Resistance Loci in Soybean with Restriction Fragment Length Polymorphism Markers

  1. B. W. Diers ,
  2. L. Mansur,
  3. J. Imsande and
  4. R. C. Shoemaker
  1. Dep. of Crop and Soil Sci., Michigan State Univ., East Lansing, MI 48824

Abstract

Abstract

Phytophthora root rot, caused by Phytophthora megasperma Drechs. f. sp. glycinea T. Kuan & D.C. Erwin, is one of the most serious diseases of soybean [Glycine max (L.) Merr.]. Six loci with alleles giving race-specific resistance of soybean to phytophthora have been reported. The first objective of this study was to map the phytophthora resistance (Rps) loci using restriction fragment length polymorphism (RFLP) markers. The second objective was to map the Rj2 locus for ineffective nodulation with RFLP markers, because of the linkage of Rj2 to Rps2. The mapping was conducted using a series of ‘Williams’ near-isogenic lines (NILs) with each having one or two phytophthora resistance alleles. The NILs were screened with 141 mapped RFLP markers. At least one polymorphism was found between each NIL and the recurrent parent. Linkage tests among the polymorphic RFLP markers, Rps loci, and the RJ2 locus were conducted using F2 populations. Linkage was found between RFLP markers and Rpsl, Kps2, Rps3, Rps4, Rps5, and RJ2. Linkage was also found between Rps2 and Rj2.

Joint contribution of North Central Region, USDA-ARS, and Journal Paper no. J-14382 of the Iowa Agric. and Home Economics Exp. Stn., Ames, IA 5001; Projects no. 2763, 2475, 2869, and 2888. This work was supported in part by Iowa State Biotechnology grant no. 480-46-09 and the Iowa Soybean Promotion Board.

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