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This article in CS

  1. Vol. 33 No. 2, p. 243-248
     
    Received: Mar 26, 1992
    Published: Mar, 1993


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doi:10.2135/cropsci1993.0011183X003300020005x

Phytoalexin Assay in Juvenile Sorghum: An Aid to Breeding for Anthracnose Resistance

  1. Abdou Tenkouano,
  2. Fred R. Miller,
  3. Gary E. Hart,
  4. Richard A. Frederiksen and
  5. Ralph L. Nicholson 
  1. D ep. of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843
    D ep. of Botany and Plant Pathology, Purdue Univ., West Lafayette, IN 47907

Abstract

Abstract

The sorghum [Sorghum bicolor (L.) Moench] plant produces antimicrobial phytoalexins that are essential components of its defense mechanism against attempted infection by Colletotrichum graminicola (Ces.) G.W. Wils., the causal agent of anthracnose. Sorghum phytoalexins are 3-deoxyanthocyanidin flavonoids shown to be luteolinidin, apigeninidin, and a caffeic acid ester of arabinosyl 5-O-apigeninidin. Crosses were made between resistant and susceptible sorghum cultivars to examine the genetic × isolate relationships of the phytoalexin response. Plants were grown in a growth chamber and in the greenhouse and inoculated with two virulent isolates and one avirulent isolate of C. graminicola 7, 21, 35, 49, and 63 d after emergence. Luteolnidin concentration was greater than that of the other phytoalexins in juvenile tissues; however, the ratios of the three compounds changed as the tissues aged and extractable amounts in tissues older than 21 d were lower than in juvenile tissues. All three phytoalexins accumulated to greater concentrations in resistant inbreds and resistant × susceptible F1 progenies than in susceptible inbreds. These relationships were unaffected by the developmental stage of the host. Thus, analysis of phytoalexins in juvenile plants appears to be a potential tool for identification and incorporation of host resistance to C. graminicola in adult plants.

Journal Article no. 30500 of the Texas Agric. Exp. Stn. and Journal Article no. 13313 of the Purdue Univ. Agric. Exp. Stn. From dissertation of senior author in partial fulfillment of Ph.D. requirements at Texas A&M Univ.

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