Generation of Haploids in Maize: A Modification of the Indeterminate Gametophyte (ig) System
- Bryan Kindiger and
- S. Hamann
Haploids in maize, Zea mays L., are relatively infrequent, but have continually been considered potential tools for the development of fixed inbred lines or as vehicles of cytoplasmic transfer. This study was undertaken to develop an efficient method for the production of androgenetic haploids in maize. A novel modification of the indeterminate gametophyte (ig) system was developed and the resulting frequency of androgenetic haploids investigated. The method combines the traditional genetic behavior of the ig allele with a tertiary trisomic (3 3 B--3Ld) stock in modified W23 background. Maintenance and generation of ig homozygotes are greatly simplified. The frequency of haploid generation has been elevated to as high as 8% utilizing inbreds of Lancaster heritage. The C, S, SD, Vg, ME, MY, CA, L, and Q cytoplasmic male-sterile stocks, homozygous for ig and of similar background to the tertiary trisomic stock, are also discussed. Development of a 3(ig) 3(ig) B-3Ld(Ig) tertiary trisomic stock in an improved modified W23 background should simplify the production of androgenetic haploids and facilitate the exchange of male-sterile cytoplasm into inbred lines of maize. This stock has potential use in experiments involving wide hybridizations between maize, teosinte [Zea mays subsp, mexicana (Schrader) Iltis], and eastern gamagrass [Tripsacum dactyloides (L.) L.]Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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